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目的 观察血管紧张素 - (1- 7) [Ang- (1- 7) ]对二肾一夹 (2 K1C)高血压大鼠肾组织转化生长因子 β1 (TGF-β1 )及其受体的影响。方法 采用微渗泵植入技术 ,建立 Ang- (1- 7)对高血压大鼠干预模型 ,光镜下观察肾脏纤维化 ,免疫组化法检测肾组织转化生长因子β1 (TGF-β1 ) ,RT- PCR检测肾组织内 TGR-β1 、TGF-β1 受体 m RNA水平。结果 Ang-(1- 7)能减轻 2 K1C高血压大鼠肾纤维化 ,减少 2 K1C高血压大鼠肾组织 TGF- β1 的蛋白表达 ,降低肾组织内 TGF- β1 、TGF- β1 受体 m RNA水平。结论 Ang- (1- 7)能减轻 2 K1C高血压大鼠肾纤维化 ,其机制之一是通过减少肾组织内TGF- β1 及其受体的表达而实现。
Objective To investigate the effects of angiotensin - (1 - 7) [Ang- (1-7)] on the expression of transforming growth factor β1 (TGF-β1) and its receptor in renal tissue of 2 K1C hypertensive rats . Methods Microinvasive implantation technique was used to establish an intervention model of Ang- (1-7) in hypertensive rats. Renal fibrosis was observed under light microscope. Transforming growth factor-β1 (TGF-β1) The levels of TGR-β1 and TGF-β1 receptor m RNA in renal tissues were detected by RT-PCR. Results Ang- (1-7) alleviated renal fibrosis in 2 K1C hypertensive rats and decreased the protein expression of TGF-β1 in renal tissue of 2 K1C hypertensive rats, and decreased the expression of TGF-β1, TGF-β1 receptor m RNA level. Conclusions Ang- (1-7) can reduce renal fibrosis in 2 K1C hypertensive rats. One of the mechanisms is that it can reduce the expression of TGF-β1 and its receptor in renal tissues.