基于基因组结构变异构建萝卜种质资源分子身份证

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萝卜种质资源类型多样,难以通过表型评价判定其亲缘关系.本试验整合4份萝卜全基因组重测序数据和520份萝卜简化基因组测序数据进行生物信息学分析,鉴定了萝卜群体基因组水平的结构变异,开发了24个通用性的分子标记.使用上述标记对255份萝卜种质进行基因分型,构建了相应的萝卜分子身份证.聚类分析结果表明,在相关系数0.55处可将255份萝卜种质分为4个类群.基于基因组水平的大片段插入/缺失开发分子标记,具有通用性高、成本低、检测方便等优点,可以促进萝卜的种质纯度鉴定及分子育种工作.
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