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目的:观察红花黄色素(SY)对Aβ_(1-42)海马注射致痴呆大鼠学习及记忆的影响,探讨红花黄色素对痴呆大鼠脑组织中炎症因子释放的调节作用。方法:Wistar大鼠60只随机分为6组,假手术组、模型组、红花黄色素10mg/kg、30mg/kg、100mg/kg组、多奈哌齐0.5mg/kg组。建立Aβ_(1-42)双侧海马注射致痴呆大鼠模型,造模后连续给药4周,至行为学测试结束。Morris水迷宫实验和跳台实验检测痴呆大鼠的空间学习记忆能力的变化;HE染色观察大脑皮层形态学改变;试剂盒法测定大鼠海马、皮层组织中i NOS、IL-1β、IL-6和TNF-α的含量。结果:与假手术组比较,模型组大鼠学习记忆能力明显下降;白介素-1β、白介素-6、肿瘤坏死因子α和诱导型一氧化氮合酶水平明显增加。经红花黄色素治疗后,红花黄色素10、30、100mg/kg学习记忆能力显著改善;HE染色结果显示,红花黄色素10、30、100mg/kg可减少神经元丢失数量,改善神经元形态异常;其对皮层IL-1β、IL-6、TNF-α和海马IL-6、i NOS的表达下调有显著性差异,同时其对海马TNF-α的表达下调也有明显的差异。红花黄色素10、30mg/kg对皮层中i NOS的表达下调有显著差异,红花黄色素30,100mg/kg对海马中IL-1β的表达下调有显著差异。结论:红花黄色素对Aβ_(1-42)海马注射致痴呆大鼠学习记忆障碍有显著改善作用,可能与其减少炎症因子释放有关。
Objective: To observe the effect of safflower yellow (SY) on the learning and memory of dementia rats induced by Aβ 1-42 hippocampus injection, and to explore the regulation of safflor yellow on the release of inflammatory cytokines in the brain of dementia rats. Methods: Sixty Wistar rats were randomly divided into 6 groups: sham operation group, model group, safflower yellow dye 10mg / kg, 30mg / kg, 100mg / kg group and donepezil 0.5mg / kg group. A rat model of dementia induced by bilateral hippocampal injection of Aβ 1-42 was established and administered continuously for 4 weeks after model establishment until the behavioral test was completed. Morris water maze test and jumping test were used to detect the spatial learning and memory abilities of dementia rats. The morphological changes of cerebral cortex were observed by HE staining. The expressions of iNOS, IL-1β, IL-6 and cortisol in hippocampus and cortex TNF-α content. Results: Compared with the sham operation group, the learning and memory abilities of the model group decreased significantly. The levels of interleukin - 1β, interleukin - 6, tumor necrosis factor - α and inducible nitric oxide synthase were significantly increased. After treated by safflower yellow, the learning and memory abilities of safflower yellow pigment at 10, 30 and 100 mg / kg were significantly improved. HE staining showed that the safflower yellow pigment at 10, 30 and 100 mg / kg could reduce the number of neurons lost and improve the nerve There was a significant difference in the expression of IL-1β, IL-6, TNF-α and IL-6, iNOS in hippocampus. There was a significant difference in iNOS expression in the cortex between safflower yellow pigment 10,30mg / kg and safflower yellow pigment 30,100mg / kg. There was a significant difference in the down-regulation of IL-1β in hippocampus. CONCLUSION: Safflower yellow can significantly improve learning and memory impairment in rats with dementia induced by Aβ_ (1-42) injection, which may be related to the decrease of inflammatory cytokines.