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目的:已有研究发现几种螺杆菌与某些动物肝脏疾病相关,本研究目的旨在探讨螺杆菌感染是否与人类肝癌的发生相关.方法:选取经病理诊断的肝癌患者15例作为研究对象,非肝癌患者13例作对照.用聚合酶链反应(PCR)扩增肝组织螺杆菌16SrRNA来检测螺杆菌,扩增产物用Southern杂交证实,并进行测序及同源比较.结果:60%(9/15)肝癌患者肝组织中发现螺杆菌存在,而非肝癌组无1例阳性(P<0.01);所有PCR阳性产物用Southern杂交得到证实;4例测序及同源比较显示,肝癌组织中的螺杆菌与幽门螺杆菌有99%的同源性.结论:肝癌患者肝组织存在螺杆菌感染,他与肝癌的发生可能存在某种联系.其是肝癌的致病因素抑或伴发感染,需进一步的研究确定.由于是扩增16SrRNA的部分序列,故目前仍不能明确是哪一种螺杆菌.
OBJECTIVE: It has been found that several Helicobacter species are associated with liver diseases in some animals, and the purpose of this study is to explore whether Helicobacter infection is associated with the development of human hepatocellular carcinoma.Methods: Fifteen patients with HCC diagnosed by pathology were selected as the research object, Thirteen non-HCC patients were used as controls, and Helicobacter hepaticus was detected by polymerase chain reaction (PCR) amplification of 16S rRNA of Helicobacter pylori. The amplified products were confirmed by Southern blotting and sequencing and homology comparison.Results: 60% (9 / 15) The presence of Helicobacter was found in the liver tissue of patients with liver cancer, but not in the group of liver cancer (P <0.01). All PCR positive products were confirmed by Southern blotting. Sequencing and homologous comparison showed that in the liver cancer tissues Helicobacter and Helicobacter pylori have 99% homology.Conclusion: The existence of Helicobacter pylori infection in liver tissue of patients with hepatocellular carcinoma may have some connection with the occurrence of hepatocellular carcinoma, which is the causative factor of hepatocellular carcinoma or concomitant infection, Since it is a partial sequence of 16SrRNA amplification, it is still not clear which one is Helicobacter.