A Comparative Analysis of Embryo and Endosperm Proteome from Seeds of Jatropha curcas

来源 :Journal of Integrative Plant Biology | 被引量 : 0次 | 上传用户:tonerzhang
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Jatropha curcas is an important economic plant for biodiesel,which is extracted mainly from the endosperm of its mature seeds.Despite the morphological and functional differences between the embryo and endosperm,proteomic characteristics of the two tissues are not yet known.Similar proteomic profiles were observed in the two-dimensional gel electrophoresis maps from the two tissues.There were 380 and 533 major protein spots in the embryo and endosperm,respectively.Fourteen identical spots,showing a notable change,were selected and identified by tandem mass spectrometry.Among these proteins,dihydrolipoamide acetyltransferase(spot 27) participates in tricarboxylic acid cycle,which is an amphibolic pathway.The two parts both included proteins related to stress(spots 8,115,118,125,130) and signal transduction(spots 7,100,108).According to the volume percentage of proteins in embryo and endosperm,the proteins in endosperm(spots 54,61,73) were catabolism-related enzymes and reserves to provide the nutrition for seed germination;the proteins in embryo(spots 27,62,122) were inclined to anabolism and utilized the nutrition from the endosperm to generate a new life. Jatropha curcas is an important economic plant for biodiesel, which is extracted mainly from the endosperm of its mature seeds. Idepite the morphological and functional differences between the embryo and endosperm, proteomic characteristics of the two tissues are not yet known. Similar proteomic profiles were observed in the two-dimensional gel electrophoresis maps from the two tissues. There were 380 and 533 major protein spots in the embryo and endosperm, respectively. Fourteen identical spots, showing a notable change, were selected and identified by tandem mass spectrometry. Among these proteins , dihydrolipoamide acetyltransferase (spot 27) participates in tricarboxylic acid cycle, which is an amphibolic pathway. two parts both proteins involved related stress (spots 8,115,118,125,130) and signal transduction (spots 7,100,108). Acording to the volume percentage of proteins in embryo and endosperm, the proteins in endosperm (spots 54, 61, 73) were catabolism-related enzymes and reserves to provi de the nutrition for seed germination; the proteins in embryo (spots 27, 62, 122) were inclined to an abolism and utilized the nutrition from the endosperm to generate a new life.
其他文献
目前我国医院单位都在面临着改革与转型,所以对于政工人员相关工作诺来讲这种全新的产业发展形式是一种挑战.为了全面落实党群思想政治工作的内容,加强对党群干部的培养提升
“四个全面”战略布局在党的十八大历届全会中得到完善发展,并最终由党中央总结提出.这一发展战略全面展现了党对于社会发展客观条件的充分分析研究与推动时代进步的大局观与
远程教育依托网络环境而存在,而在纷繁复杂的网络世界里进行思想政治教育,其难度自然是非常大的.据此,本文分析远程教育环境下思想政治教育的困难及措施,以期能为构建现代化
中央人民广播电台的《全国新闻联播》,从创立到现在已经过了60多个年头。在听众的心目中,它有一张“成熟”的脸,报道庄重成熟、有深度和广度。但我觉得它还应该有一颗“年轻
面对一个巨大的增长机遇,生产管理应如何迅速满足发展,在资源输入和输出的转化过程中,有效地通过战略决策、流程整合和人员素质提升来带动经济效益,使企业成为客户发展不可或缺的合作伙伴,已成为企业核心竞争力的重要战略。 纵观生产管理的发展历史,本世纪初叶,被誉为“科学管理之父”的美国工程师泰罗首倡科学管理运动,将科学的定量分析方法,引入到生产管理之中,制定了科学的作业程序和标准,成功地揭开了生产管理
11月27日晚,在北京国家大剧院举行了由中国文联、文化部、中国音协、中国国家交响乐团、中国交响乐发展基金会主办的“祝贺韩中杰90华诞暨从艺73周年专场音乐会”。原中央政
经济改革给中国社会带来了前所未有的变化,也吸引了国内外许多专家学者对其发展的奥妙进行探讨。核心竞争力概念的提出是“资源观企业理论”发展的结果,在20世纪90年代初,不少学
我国煤炭企业在人力资源规划管理方面有了较好发展。但是煤炭企业在人才规划方面具有规划与现实脱节,内部人才不平衡不充分,甚至出现没有起到引导企业人力资源未来发展的作用;严
论文将企业价值的评估方法和理论应用于财务分析的过程中,以新钢钒股份有限公司的财务报表及相关资料为依据,在分析新钢钒股份有限公司基本情况的基础上,以财务能力的分析为基础
基于电流走向的电气设备故障诊断方法可以明确可疑的故障点,再结合电路图进行对比分析,就能实现对故障点的精确定位和快速排除,是一种行之有效的手段.本文对该方法在汽车空调