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目的观察再生障碍性贫血(再障)患儿血辅助性T淋巴细胞17(Th17)的数量及功能,探讨Th17在再障发病中的作用。方法研究对象为新发再障患儿25例(再障组),选取非血液系统疾病患儿10例为对照组。采用流式细胞术检测2组患儿骨髓单个核细胞IL-23受体(IL-23R)+CD4+/CD4+比例,ELISA法测定骨髓液上清IL-6、IL-23和IL-17水平,分析IL-23R+ CD4+/CD4+比例。结果再障组IL-23R+ CD4+/CD4+比例为(3.5±2.9)%,明显高于对照组[(1.6±1.0)%](P<0.05)。再障组骨髓液上清IL-6、IL-23和IL-17水平分别为(23.2±11.3)μg·L-1、(2.0±0.7)μg·L-1和(2.1±1.1)μg·L-1,明显高于对照组[(4.2±1.3)μg·L-1、(1.1±0.7)μg·L-1、(1.3±0.2)μg·L-1](Pa<0.05)。结论再障患儿Th17细胞数量增加,功能亢进,辅助性T淋巴细胞17参与再障的发病。
Objective To investigate the number and function of blood-helper T-lymphocytes 17 (Th17) in children with aplastic anemia (aplastic anemia) and to explore the role of Th17 in the pathogenesis of aplastic anemia. Methods 25 newborns with aplastic anemia (Aplastic anemia) were included in the study. Ten children with non-hematologic disorders were selected as the control group. The proportion of IL-23R + CD4 + / CD4 + in bone marrow mononuclear cells was measured by flow cytometry. The levels of IL-6, IL-23 and IL-17 in bone marrow fluid supernatant were measured by ELISA. The IL-23R + CD4 + / CD4 + ratio was analyzed. Results The percentage of IL-23R + CD4 + / CD4 + in the AA group was (3.5 ± 2.9)%, significantly higher than that in the control group (1.6 ± 1.0%) (P <0.05). The levels of IL-6, IL-23 and IL-17 in the bone marrow fluid supernatant of the AA group were (23.2 ± 11.3) μg · L-1, (2.0 ± 0.7) μg · L-1 and (2.1 ± 1.1) μg · L-1 was significantly higher than that in the control group [(4.2 ± 1.3) μg · L-1, (1.1 ± 0.7) μg · L-1, (1.3 ± 0.2) μg · L-1], respectively. Conclusions The number of Th17 cells in children with aplastic anemia is increased and their functions are hyperactive. In addition, helper T lymphocytes 17 are involved in the pathogenesis of aplastic anemia.