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为了解肺炎衣原体 (Cpn)感染与小儿哮喘之间的关系 ,并探讨哮喘患儿Cpn感染的诊断及治疗 ,采用微量免疫荧光(MIF)试验及巢式聚合酶链式反应 (nPCR)2种方法对45例哮喘发作期患儿及20例正常对照组进行Cpn检测。结果 :①哮喘组14例nPCR检测阳性 ,对照组1例阳性 ,两者相比差异有显著性 (P<0.05) ,表明Cpn感染与小儿哮喘发作密切相关。②14例nPCR检测阳性患儿在随后的发作中5例 (35.71 %)仍为阳性 ,表明哮喘患儿中慢性或重复Cpn感染较常见。③就诊或住院后5例Cpn感染哮喘患儿接受了大环内酯类抗生素治疗 ,其中3例病情缓解 ,表明对Cpn感染哮喘患儿进行抗Cpn治疗是必要的。④经全自动荧光测序 ,随机抽取的2例阳性标本和Cpn标准株 (CWL_29)的nPCR产物DNA序列完全一致。
To understand the relationship between Chlamydia pneumoniae (Cpn) infection and pediatric asthma and to explore the diagnosis and treatment of Cpn infection in children with asthma, two kinds of methods, MIF assay and nested polymerase chain reaction (nPCR) 45 cases of asthma attack in children and 20 normal control group for Cpn detection. Results: ① The positive expression of nPCR was detected in 14 cases of asthma group and 1 case in the control group (P <0.05), indicating that Cpn infection is closely related to the onset of asthma in children. In 14 cases of nPCR positive children in the subsequent seizures in 5 cases (35.71%) was still positive, indicating that children with asthma chronic or repeated Cpn infection is more common. ③ After treatment or hospitalization, 5 cases of Cpn-infected asthmatic children received macrolide antibiotics, of which 3 cases of remission, indicating that Cpn-infected children with anti-Cpn therapy is necessary. ④ The DNA sequences of nPCR products of two positive samples and Cpn standard strains (CWL_29) were completely identical by automatic fluorescence sequencing.