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目的探讨980 nm红外连续激光对金属医疗器械表面高效灭菌的功率和时间阈值关系。方法采用人工污染实验法,以无菌钢制手术刀为载体,用金黄色葡萄球菌和大肠杆菌分别进行污染,经不同功率、对应不同辐照时间的激光消毒灭菌后用细菌培养基清洗刀片采样,采用增菌培养来检测红外激光对大肠杆菌和金黄色葡萄球菌的灭活情况。结果 980 nm激光在5.24 W时辐照29 s,6.87 W时辐照24 s,8.33 W时辐照14 s,10.11 W时辐照14 s都能彻底杀死金黄色葡萄球菌;980 nm激光在5.24 W时辐照22 s,6.87 W时辐照12 s,8.33 W时辐照11 s,10.11 W时辐照9 s都能彻底杀死大肠杆菌。大肠杆菌比金黄色葡萄球菌更易灭活。依据实验数据拟合,在同一激光功率下,两种细菌存活率与辐照时间的函数符合一阶反应动力学形式。结论 980 nm红外激光能够实现金属医疗器械表面的快速消毒灭菌,并且功率越高则时间越短、效果越好。
Objective To investigate the relationship between power and time threshold for efficient sterilization of metal medical devices by 980 nm infrared continuous laser. Methods Artificial pollution test was used to treat the stainless steel scalpel with Staphylococcus aureus and Escherichia coli respectively. The bacteria were sterilized with different power and different irradiation time, then cleaned with a bacterial culture medium Sampling, the use of enrichment culture to detect infrared laser inactivation of Escherichia coli and Staphylococcus aureus. Results The 980 nm laser irradiation for 29 s at 5.24 W, the 24 s irradiation at 6.87 W, the 14 s irradiation at 8.33 W and the 14 s irradiation at 10.11 W killed 980 S. laser at Irradiation at 5.24 W for 22 s, irradiation at 6.87 W for 12 s, irradiation at 8.33 W for 11 s and irradiation at 10.11 W for 9 s completely killed E. coli. Escherichia coli more easily inactivated than Staphylococcus aureus. According to the experimental data fitting, under the same laser power, the function of two kinds of bacteria survival rate and irradiation time conforms to the first-order reaction kinetics. Conclusion 980 nm infrared laser can quickly sterilize the surface of metal medical devices, and the higher the power, the shorter the time, the better the effect.