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目的:观察中药银翘散体内抑制甲1型流感病毒FM1株复制的作用。方法:以甲1型流感病毒FM1鼠肺适应株滴鼻感染BALB/c小鼠,并用病毒唑及高、中、低剂量银翘散干预相应组别小鼠,观察生存状态及检测肺指数,以苏木素-伊红染色(HE染色)法观察病理改变;将鼠肺悬液以空斑减少试验定量检测感染性病毒滴度,蛋白免疫印迹技术检测核蛋白(nucleoprotein,NP)表达。结果:银翘散可以减轻全身症状,降低肺指数;减少鼠肺病变范围及肺泡腔渗出物;降低鼠肺病毒滴度及鼠肺流感病毒NP表达量。结论:银翘散有体内抑制甲1型流感病毒FM1株复制的作用。
OBJECTIVE: To observe the effect of Yinqiaoqiao on inhibiting the replication of FM1 strain of influenza A virus type 1 in vivo. Methods: BALB / c mice were intranasally inoculated intranasally with FM1 strain of lung virus of influenza A virus type 1 and the corresponding groups of mice were treated with ribavirin and high, medium and low doses of Yinqiaoqiao. The survival status, lung index, The pathological changes were observed by hematoxylin-eosin staining (HE staining); the infectious lung virus titer was detected by plaque reduction assay in rat lung suspension, and the expression of nucleoprotein (NP) was detected by Western blotting. Results: Yinqiao powder can reduce systemic symptoms and reduce the index of lungs; reduce the range of rat lung lesions and alveolar exudate; reduce the mouse lung virus titer and the expression of mouse lung influenza virus NP. Conclusion: Yinqiao powder can inhibit the replication of FM1 strain of influenza A virus type 1 in vivo.