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将含有魔芋AGPase反义基因的质粒pBAGP通过冻融法转化到根癌农杆菌(Agrobacterium tumefaciens)菌株LBA4404中,再采用叶盘法将其转化进番茄(Lycopersicon esculentumMill.)栽培品种“合作908”中,获得含AGPase基因的番茄抗性植株。最后,经卡那抗性鉴定、NPTⅡ基因和AGPase基因PCR扩增和PCR-Southern杂交检测表明,反义AGPase基因成功整合到番茄基因组,为番茄改良品质育种奠定了材料基础。
The plasmid pBAGP containing antisense gene of konjac AGPase was transformed into Agrobacterium tumefaciens strain LBA4404 by freeze-thawing method and then transformed into Lycopersicon esculentum Mill cultivar “Cooperative 908 ”To obtain tomato-resistant plants containing the AGPase gene. Finally, the kanamycin resistance assay, PCR amplification of NPTⅡ gene and AGPase gene and PCR-Southern blot showed that the antisense AGPase gene was successfully integrated into tomato genome, which laid the material foundation for improved quality breeding of tomato.