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以实验室纯种培养的赤潮异弯藻为对象,研究了赤潮异弯藻毒素的产生部位,分析了藻毒素浓度与溶血活性的相关性,并对理化因子对藻毒素溶血活性的影响进行了探讨。结果表明,去藻过滤液、细胞内含物、细胞再悬液和细胞碎片的百分溶血度分别为35.68%、65.62%、88.23%和83.21%,细胞再悬液和细胞碎片的溶血活性显著高于其他组分,赤潮异弯藻溶血毒素主要来自于细胞内和细胞结构物质。赤潮异弯藻毒素存在浓度相关的溶血性,但不存在光敏感性,并在中性偏酸时溶血活性最高。Mg2+、Zn2+、Cu2+抑制了溶血活性,Ca2+、Mn2+、Co2+对溶血活性的抑制作用不显著。金属鳌合剂EDTA对赤潮异弯藻毒素的溶血活性没有明显的影响。
In this study, the production of heterotaxic algae toxin in red tide was studied in laboratory, and the correlation between the concentration of algal toxin and hemolytic activity was analyzed. The effects of physicochemical factors on hemolysis activity of algal toxin Discussion. The results showed that the percent hemolysis of the filtrate, cellular contents, cell suspension and cell debris were 35.68%, 65.62%, 88.23% and 83.21%, respectively. The hemolytic activity of cell suspension and cell debris were significant Above the other components, Heterosigma akashiho hemolytic toxin mainly comes from intracellular and cellular structures. HABs have concentration-related hemolytic, but not photosensitivity, and have the highest hemolytic activity when neutral acidified. Mg2 +, Zn2 + and Cu2 + inhibited the hemolytic activity. The inhibitory effect of Ca2 +, Mn2 + and Co2 + on hemolytic activity was not significant. EDTA, a metal chelator, had no significant effect on hemolytic activity of HABs.