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为了确定饲料中胆固醇含量对雄性红螯光壳螯虾(Cherax quadricarinatus)生长和生殖的影响,本研究通过对初始体长为10 cm左右的雄虾投喂胆固醇添加量分别为对照组(0%)、实验1组(0.25%)、实验2组(0.50%)、实验3组(0.75%)、实验4组(1.00%)的人工配合饲料10周后,测定各组实验虾的肝胰腺消化酶、精巢碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)和ATP酶活力。结果显示,生长指标和胰蛋白酶活力以实验2组最高;脂肪酶活力实验2、3、4组之间无显著差异,但显著高于实验1组和对照组;精巢AKP活力和LDH活力均以实验3组最高;精巢ATP酶活力实验2组和实验3组之间无显著差异,均显著高于实验1、4组和对照组。结果表明,对处于性腺快速发育阶段的雄性红螯光壳螯虾饲料中胆固醇的最适添加量在0.50%～0.75%之间。
In order to determine the effect of dietary cholesterol on the growth and reproduction of Cherax quadricarinatus, in this study, the cholesterol levels of males fed the initial body length of 10 cm were 0% ), Experiment 1 (0.25%), experiment 2 (0.50%), experiment 3 (0.75%) and experiment 4 (1.00%) artificial diet for 10 weeks, Enzymes, testis alkaline phosphatase (AKP), lactate dehydrogenase (LDH) and ATPase activity. The results showed that the growth index and the trypsin activity were the highest in the experimental group 2; the lipase activity was no significant difference between the experimental groups 2, 3 and 4, but significantly higher than the experimental group 1 and the control group; AKP activity and LDH activity There was no significant difference between experimental group 2 and experimental group 3, which was significantly higher than that of experimental group 1 and 4 and control group. The results showed that the optimum amount of cholesterol in the feed of male Chellabora chellansis in the rapid development of gonads was between 0.50% ~ 0.75%.