论文部分内容阅读
目的探讨鼻咽癌CNE1细胞及放射抗拒细胞亚系CNE1/70R细胞在倍增时间、放射敏感性、药物敏感性、细胞凋亡、细胞周期方面的差异。方法用临床常规分割根治剂量70 Gy体外照射建立鼻咽癌放射抗拒细胞模型CNE1/70R。采用生长曲线检测CNE1及CNE1/70R两株细胞倍增时间,细胞克隆形成实验比较细胞放射敏感性,MTT法检测细胞对药物多西紫杉醇、顺铂的敏感性,流式细胞术检测细胞凋亡和周期的变化。结果 CNE1/70R和CNE1细胞的倍增时间分别为(1.612±0.069)、(1.481±0.046)d;与CNE1细胞比较,CNE1/70R细胞的放射抗拒性增强,对化疗药物多西紫杉醇和顺铂敏感性增高;细胞经照射后,CNE1细胞G_2/M期阻滞较CNE1/70R细胞更加明显;细胞经照射相同剂量后,CNE1细胞凋亡率高于CNE1/70R细胞。以上观察指标比较,两株细胞差异均有统计学差异(P均<0.05)。结论成功建立人鼻咽癌放射抗拒株CNE1/70R,且与亲代细胞在放射及药物治疗敏感性方面有稳定差异。
Objective To investigate the differences of doubling time, radiosensitivity, drug sensitivity, apoptosis and cell cycle between nasopharyngeal carcinoma CNE1 cells and radioresistant cell subline CNE1 / 70R cells. Methods Radiation-resistant cell model CNE1 / 70R of nasopharyngeal carcinoma was established by routinely divided radical dose of 70 Gy in vitro. Growth curves were used to detect the doubling time of CNE1 and CNE1 / 70R cells. Cell colony formation assay was used to compare the cell radiosensitivity. MTT assay was used to detect the sensitivity of the cells to docetaxel and cisplatin. Flow cytometry was used to detect apoptosis and Cycle changes. Results The doubling time of CNE1 / 70R and CNE1 cells were (1.612 ± 0.069) and (1.481 ± 0.046) d, respectively. Compared with CNE1 cells, CNE1 / 70R cells showed enhanced radiosensitivity and chemosensitivity to docetaxel and cisplatin The cell cycle arrest of CNE1 cells was more obvious than that of CNE1 / 70R cells after irradiation. The apoptosis rate of CNE1 cells was higher than that of CNE1 / 70R cells after irradiation of the same dose. The above indicators, the two cells were statistically significant differences (P all <0.05). Conclusion The human nasopharyngeal carcinoma cell line CNE1 / 70R is successfully established and has stable differences with the parental cells in radiosensitivity and drug therapy.