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目的比较T2DM患者、T2DM合并牙周病患者与正常对照者龈沟液(GCF)中基质金属蛋白酶-1(MMP-1)和GCF量,以及T2DM患者GCF中MMP-1量与糖代谢状况的关系。方法使用滤纸条法采集GCF。选取T2DM组、T2DM合并牙周病组各30例,正常对照组30名,ELISA测定GCF中MMP-1含量,运用高效液相色谱法检测HbA1c水平。结果 T2DM、T2DM合并牙周病组GCF量高于正常对照组[(2.81±1.58)、(3.56±2.96)vs(1.02±0.65)μl/样本](P<0.01)。T2DM合并牙周病组GCF中MMP-1量高于T2DM、正常对照组[(5.02±0.16)vs(3.01±0.09)、(2.24±0.11)μl/样本](P<0.01)。T2DM组与正常对照组GCF中MMP-1量比较差异无统计学意义(P>0.05)。T2DM合并牙周病组GCF中MMP-1量与HbA1c水平无相关性(r=0.153,P=0.151)。结论3组GCF及MMP-1水平在T2DM合并牙周病组中最高,在对照组最低,提示牙周炎症是影响结果的主要原因。MMP-1水平在T2DM组中低于T2DM合并牙周病组,提示T2DM患者不只是MMP-1水平改变而导致牙周病的易感,可能是多个因素共同作用的结果。
Objective To compare the expression of matrix metalloproteinase-1 (MMP-1) and GCF in GCF and GCF in T2DM patients with T2DM complicated with periodontal disease and normal controls and the relationship between the level of MMP-1 and glucose metabolism relationship. Method The filter paper method was used to collect GCF. Thirty patients with T2DM, 30 with T2DM complicated with periodontal disease and 30 normal controls were enrolled. The content of MMP-1 in GCF was determined by ELISA and the level of HbA1c was detected by high performance liquid chromatography. Results The GCF levels of T2DM and T2DM with periodontal disease were significantly higher than those of the control group [(2.81 ± 1.58), (3.56 ± 2.96) vs (1.02 ± 0.65) μl / sample] (P <0.01). The amount of MMP-1 in GCF of T2DM combined with periodontal disease group was higher than that of T2DM group (P <0.01), (5.02 ± 0.16) vs (3.01 ± 0.09) and (2.24 ± 0.11) μl / sample in normal control group. There was no significant difference in the amount of MMP-1 in GCF between T2DM group and normal control group (P> 0.05). There was no correlation between the level of MMP-1 and the level of HbA1c in T2DM with periodontal disease (r = 0.153, P = 0.151). Conclusions The levels of GCF and MMP-1 in the three groups are the highest in T2DM combined with periodontal disease group and the lowest in control group, suggesting that periodontal inflammation is the main reason for the effect. The level of MMP-1 in T2DM group was lower than that in T2DM group. It suggested that not only the change of MMP-1 level but also the susceptibility of periodontal disease in T2DM patients may be the result of many factors.