[目的]为一品红原生质体融合及遗传转化等相关研究奠定基础。[方法]以一品红品种“喜庆红”的顶芽和带芽茎段为外植体,进行愈伤组织诱导、增殖及悬浮系建立的研究。[结果]以浓度0.1%升汞处理顶芽和带芽茎段的时间分别以6 min和10 min为宜。在MS固体培养基里分别添加活性炭、PVP和Vc后,愈伤组织在培养过程中褐化程度有所减轻,但与对照相比差异并不显著。[结论]一品红愈伤组织悬浮系的适合继代周期为7~8 d。 [Objective] The research aimed to lay the foundation for the related research on the protoplast fusion and genetic transformation of poinsettia. [Method] With top bud and bud stem segments of poinsettia variety “happy red ” as explants, the establishment of callus induction, proliferation and suspension system was studied. [Result] The time of treating the top bud and bud with buds with 0.1% mercuric chloride was 6 min and 10 min, respectively. After adding activated carbon, PVP and Vc respectively in MS solid medium, the degree of browning of callus in the culture process was reduced, but the difference was not significant compared with the control. [Conclusion] The suitable subculture cycle of poinsettia callus suspension system was 7-8 days.