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目的:观察肝内型门脉高压大鼠门静脉超微结构及生物力学特性,为进一步探索门脉高压病理机制提供实验依据.方法:四氯化碳皮下注射诱导肝内型门静脉高压大鼠模型,生物力学技术测定门脉高压形成中门静脉在体直径、纵向拉伸应力-应变曲线及残余应力大小,透视电镜下观察门静脉超微结构.结果:与正常组大鼠相比,门脉高压组大鼠门静脉体外直径显著增粗(1.53 mm±0.28 mm vs 1.14 mm±0.17 mm,P<0.01);门静脉超微结构发生显著改变;纵向拉伸应力-应变曲线较陡峭,整个拉伸应变过程耗时短,50%应变时应力是1.04 N±0.13 N,显著大于正常组(0.51N±0.08 N,P<0.01);门静脉张开角的弧度及组内波动幅度较正常组显著增大(110.34度±8.09度vs 79.93度±2.44度,P<0.01).结论:肝内型门脉高压大鼠门静脉管壁存在异常重构,其顺应性下降,门静脉呈周向和轴向高应力的生物力学特性改变.
OBJECTIVE: To observe the ultrastructure and biomechanical properties of portal vein in rats with intrahepatic portal hypertension to provide experimental evidence for further exploring the pathological mechanism of portal hypertension.METHODS: Intrahepatic portosystemic Hypertension rat model was induced by subcutaneous injection of carbon tetrachloride Biomechanical technique was used to measure the diameter and longitudinal tensile stress-strain curves and the residual stress of portal vein in portal hypertension, and the ultrastructure of portal vein was observed under electron microscope.Results: Compared with normal group, portal hypertension group In vitro rat portal vein diameter was significantly thicker (1.53 mm ± 0.28 mm vs 1.14 mm ± 0.17 mm, P <0.01); portal vein ultrastructure was significantly changed; longitudinal tensile stress-strain curve steep, the entire tensile strain time-consuming process The stress at the 50% strain was 1.04 N ± 0.13 N, significantly higher than that in the normal group (0.51 N ± 0.08 N, P <0.01). The radian of opening angle of the portal vein and the amplitude of the group fluctuation were significantly higher than those of the normal group (110.34 ± 8.09 Degrees vs 79.93 degrees ± 2.44 degrees, P <0.01) .Conclusion: The portal vein wall of intrahepatic portal hypertensive rats is abnormally remodeled and its compliance is decreased. The biomechanical properties of the portal vein in circumferential and axial high stress are changed .