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目的 研究二硫化碳 (CS2 )对卵母细胞和受精卵雌原核染色体非整倍体率的影响。方法 用CS2 给成年雌性NIH小鼠一次性灌胃 (372、744、1488mg/kg)和多次吸入 (199、6 5 1、12 0 9mg/m3)染毒 ,灌胃后雌、雄鼠以 1∶1同笼过夜 ,收集卵母细胞和受精卵作细胞遗传学分析 ,检测非整倍体率。结果 CS2 一次性灌胃 (372、744、1488mg/kg)和多次吸入 (199、6 5 1、12 0 9mg/m3)诱导的小鼠MⅡ卵母细胞染色体非整倍体率分别为 4.88%、6 .82 %、6 .82 %和 6 .6 0 %、12 .0 0 %、10 .0 0 % ,与对照组(0 .78%、1.30 % )比较 ,差异有显著性 (P <0 .0 5 )。CS2 一次性灌胃诱导的小鼠受精卵雌原核染色体非整倍体率为 2 .18%、6 .98%、7.40 % ,后两组与对照组 (1.0 2 % )比较 ,差异亦有显著性 (P <0 .0 5 )。结论 接触CS2 可诱导小鼠MⅡ卵母细胞和受精卵雌原核染色体非整倍体率增高
Objective To investigate the effects of carbon disulfide (CS2) on the rate of aneuploidy in the prokaryotic chromosomes of oocytes and fertilized eggs. Methods Adult female NIH mice were treated with CS2 once (372,744,1488 mg / kg) and multiple inhalation (199,651,1209 mg / m3) 1: 1 overnight with the cage, collecting oocytes and fertilized eggs for cytogenetic analysis, detection of aneuploidy rate. Results The chromosomal aneuploidy rates of mouse M Ⅱ oocytes induced by CS2 (372,744,1488 mg / kg) and multiple inhalation (199,651 and 1209 mg / m3) were 4.88% , 6 .82%, 6 .82% and 6. 60%, 12. 0%, 10. 0%, respectively, compared with the control group (0.78%, 1.30%), the difference was significant (P < 0 .0 5). The rate of aneuploidy in female fertilized eggs induced by CS2 was 2.18%, 6.98% and 7.40%, respectively. The differences between the latter two groups and the control group (1.02%) were also significant Sex (P <0.05). Conclusion Exposure to CS2 induces an increase in the aneuploidy rate of estrogen-like chromosomes in mouse MⅡ oocytes and fertilized eggs