在细胞培养实验中,血清的处理和细胞的冻存这两方面的问题,是较为复杂也是比较容易出错的。而血清处理和细胞冻存过程中的技术细节若处理不当,会对细胞培养的效果产生较明显的影响。就理论学习及实验操作中获得的经验,对血清的保存、解冻、沉淀物和热灭活的问题,及细胞冻存的方法和注意事项等细节问题作一总结。 In cell culture experiments, problems with both serum handling and cell cryopreservation are more complicated and error-prone. However, if the technical details of serum treatment and cell cryopreservation are not properly handled, the effect of cell culture will be significantly affected. On the theoretical learning and experimental experience gained in the practice, the preservation of serum, thawing, precipitation and heat inactivation problems, and cell freezing methods and precautions details such as a summary.