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目的了解WU多瘤病毒(WUPyV)在小儿呼吸道感染时其急性期外周血细胞因子的变化,以探讨该病毒感染的免疫致病机制。方法应用PCR技术对儿科急性呼吸道感染1 253例住院患儿的咽拭子及82例健康体检儿童咽拭子进行WUPyV核酸检测,并同时检测呼吸道合胞病毒(RSV)、人类博卡病毒(HBoV)、人偏肺病毒(hMPV)、腺病毒(ADV)、流感病毒A、B型(IFV)、副流感病毒1、3型(PIV)和鼻病毒(Rhv)共10种病毒。采用液相蛋白芯片技术同时对15例单纯WUPyV感染患儿、20例单纯RSV感染患儿、22例正常对照儿童外周血血清中12种细胞因子(IFN-γ、IL-1β、IL-1α、IL-2、IL-4、IL-6、IL-8、IL-10、IL-12p70、IL-12p40、IL-13、TNF-α)的含量进行检测。结果 1253例患儿WUPyV阳性32例(占2.55%),其中单纯WUPyV感染15例(46.88%),合并其他病毒感染的有17例(53.13%),分别为RSV、RHV、IVA、ADV;RSV阳性158例(占12.61%),其中单纯RSV感染85例,合并感染73例,与另9种病毒均存在混合感染情况。82例健康体检儿童10种病毒的PCR检查均阴性。单纯WUPyV组患儿外周血IL-6、IL-8、IL-1β、TNF-α、IL-12p40水平较正常对照组明显升高(P均<0.05),单纯RSV组患儿外周血IL-6、IL-8、IL-1β、TNF-α、IL-12p40水平也较正常对照组明显升高(P均<0.01),单纯WUPy组此5种细胞因子较正常对照组升高的程度均较单纯RSV组稍低,但两病毒组间此5种细胞因子差异无统计学意义(P均>0.05)。单纯WUPyV组及单纯RSV组患儿外周血IL-2、IL-4、IL-1α、INF-γ水平均稍高于对照组,但差异无统计学意义(P>0.05)。3组儿童外周血中几乎未检测到IL-10、IL-12p70、IL-13。结论 WUPyV感染可导致患儿免疫功能异常,其模式与RSV组感染导致的异常细胞因子改变类似但程度较轻。
Objective To investigate the changes of peripheral blood cytokines in acute phase of Wu polyoma virus (WUPyV) in children with respiratory tract infection, in order to investigate the immunopathogenesis of WUPyV infection. Methods WUPyV nucleic acid was detected in throat swabs of 1 253 pediatric hospitalized children with pediatric acute respiratory infection and 82 healthy children with throat swabs by polymerase chain reaction and simultaneous detection of respiratory syncytial virus (RSV), human Boka virus (HBoV) ), Human metapneumovirus (hMPV), adenovirus (ADV), influenza A, B (IFV), parainfluenza virus type 1 (PIV) and rhinovirus (Rhv). Using liquid protein chip technique, the levels of 12 cytokines (IFN-γ, IL-1β, IL-1β, IL-1β, IL- IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-12p40, IL-13, TNF- Results Among the 1253 cases, 32 cases were positive for WUPyV (2.55%), among which 15 cases (46.88%) were WUPyV infection alone and 17 cases (53.13%) were complicated with other virus infection, which were RSV, RHV, Positive 158 cases (accounting for 12.61%), of which 85 cases of pure RSV infection, 73 cases of co-infection, mixed infection with the other 9 kinds of viruses. The PCR test of 10 viruses in 82 healthy children was negative. The levels of IL-6, IL-8, IL-1β, TNF-α and IL-12p40 in the peripheral blood of children in the simple WUPyV group were significantly higher than those in the normal control group (all P <0.05) 6, IL-8, IL-1β, TNF-α and IL-12p40 were significantly higher than those in normal control group (all P <0.01). The levels of these five cytokines in WUPy group were higher than those in normal control group Compared with simple RSV group, it was slightly lower, but there was no significant difference between the two virus groups (P> 0.05). The levels of IL-2, IL-4, IL-1α and INF-γ in peripheral blood of WUPyV group and RSV group were all higher than those of control group, but the difference was not statistically significant (P> 0.05). IL-10, IL-12p70, IL-13 were almost not detected in the peripheral blood of 3 groups of children. Conclusions WUPyV infection can lead to abnormal immune function in children with similar but lesser degree of abnormal cytokine changes caused by infection with RSV.