[Objective] To investigate the effects of drug serum of Valeriana jatamansi on the splenic lymphocytes and peritoneal macrophages in mice.[Methods] The influence of drug serum on splenic lymphocytes transformation,metabolic level and phagocytosis of peritoneal macrophages in mice were detected by MTT method and neutral red test respectively.[Result] Within certain dose range,the single action or synergetic non-specific mitogen(Con A or LPS) effects of splenic lymphocytes and peritoneal macrophages in the drug serum group was significantly different from that of the control group.[Conclusion] The drug serum of Valeriana jatamansi could strengthen the transformation of splenic lymphocytes,improve the metabolic level and phagocytosis of peritoneal macrophages in mice,and enhance the immunity ability. [Objective] To investigate the effects of drug serum of Valeriana jatamansi on the splenic lymphocytes and peritoneal macrophages in mice. [Methods] The influence of drug serum on splenic lymphocytes transformation, metabolic level and phagocytosis of peritoneal macrophages in mice were detected by MTT method and neutral red test respectively. [Result] Within certain dose range, the single action or synergetic non-specific mitogen (Con A or LPS) effects of splenic lymphocytes and peritoneal macrophages in the drug serum group was significantly different from that of the control group [Conclusion] The drug serum of Valeriana jatamansi could strengthen the transformation of splenic lymphocytes, improve the metabolic level and phagocytosis of peritoneal macrophages in mice, and enhance the immunity ability.