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目的 了解我国妇女生殖道感染性疾病患者中生殖道是否存在新型致病支原体 (穿通支原体、发酵支原体和梨支原体 )的感染 ,为临床诊治提供依据。方法 采集女性生殖道感染患者和正常人 (各 172例 )的宫颈分泌物 ,接种在改良的SP 4培养基 ,进行分离培养。阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。结果 从 172例患者宫颈分泌物中分离到 1株穿通支原体 (0 5 8% ) ,2株发酵支原体 (1 16 % )。从 172名正常女性的宫颈分泌物中没有分离到相应的菌株。结论 从病原学首次证实 ,南京地区女性生殖道感染性疾病患者中存在穿通支原体、发酵支原体的感染。
Objective To understand whether there is a new pathogenic mycoplasma (Mycoplasma penetrans, Mycoplasma fermentum, and Mycoplasma pear) in the genital tract of women with reproductive tract infections in our country, so as to provide evidence for clinical diagnosis and treatment. Methods Cervical secretions from female genital tract infection patients and normal subjects (172 cases each) were collected and inoculated on modified SP 4 medium for isolation and culture. Positive cultures were identified by biochemical reactions, co-agglutination tests, metabolic inhibition tests, polymerase chain reaction and DNA sequencing. Results One strain of Mycoplasma penetrant (0 58%) and two strains of Mycoplasma fermentum (16%) were isolated from the cervical secretions of 172 patients. The corresponding strains were not isolated from cervical secretions of 172 normal women. Conclusions It was first confirmed from etiology that there were infections of Mycoplasma and Mycoplasma fermentum among female genital tract infectious diseases in Nanjing.