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应用UHPLC-HR-MSn技术分析检测茵陈提取物中的化学成分。采用ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.7μm)色谱柱和0.1%甲酸水溶液(A)-乙腈(B)流动相系统进行梯度洗脱;应用LTQ-Orbitrap质谱负离子模式检测茵陈提取物中的化学成分。根据精确分子量数据和多级质谱碎片离子,结合对照品比对和文献报道,共从茵陈提取物中分析鉴定了50个化学成分,包括21个黄酮、22个有机酸、6个香豆素和1个其他类成分。应用UHPLC-LTQ-Orbitrap高分辨液质联用技术较为全面地阐明了茵陈提取物中的化学成分,为进一步研究茵陈提取物的全面质量控制方法、体内代谢过程以及药效物质基础提供了科学依据。
Application of UHPLC-HR-MSn technology to analyze the chemical composition of capillaris extract. Gradient elution was performed on an ACQUITY UPLC HSS T3 (2.1 mm × 100 mm, 1.7 μm) column and a 0.1% formic acid aqueous solution (A) -acetonitrile (B) mobile phase system. The LTQ-Orbitrap negative ion mode In the chemical composition. Based on the accurate molecular weight data and multi-stage mass spectrometry fragment ions, in combination with reference materials and literature reports, a total of 50 chemical constituents, including 21 flavones, 22 organic acids and 6 coumarins And 1 other class component. The application of UHPLC-LTQ-Orbitrap high-resolution liquid chromatography-mass spectrometry (LC-MS / MS) technology to elucidate the chemical constituents of Herba Artemisiae Cichorii extract in a comprehensive way provides a basis for further research on total quality control of Herba Artemisiae extract, metabolic process in vivo and pharmacodynamic basis Scientific basis.