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目的研究RNA干扰(RNAi)及厄洛替尼阻断表皮生长因子受体(EGFR)信号途径对肺腺癌A549细胞增殖的影响。方法肺腺癌A549细胞分为对照组、RNAi组、厄洛替尼组。采用倒置相差显微镜观察EGFR信号阻断前后细胞形态的变化,RT-PCR检测EGFR mRNA表达,流式细胞术检测细胞凋亡,Western blot检测EGFR及Bcl-2、Bax表达。结果 RNAi阻断EGFR mRNA表达下调。与对照组相比,RNAi组及厄洛替尼组A549细胞凋亡增加,EGFR表达降低,Bcl-2水平表达降低,Bax蛋白表达增高,Bcl-2/Bax比值降低。结论阻断EGFR信号途径可促进肺腺癌A549细胞凋亡,其机制可能与降低Bcl-2/Bax比值水平相关。
Objective To investigate the effects of RNA interference (RNAi) and erlotinib on the proliferation of human lung adenocarcinoma A549 cells by blocking the signal transduction of epidermal growth factor receptor (EGFR). Methods Lung adenocarcinoma A549 cells were divided into control group, RNAi group and erlotinib group. The changes of cell morphology before and after the blockade of EGFR signal were observed by inverted phase contrast microscope. The expression of EGFR mRNA was detected by RT-PCR. The apoptosis was detected by flow cytometry. The expressions of EGFR, Bcl-2 and Bax were detected by Western blot. Results RNAi blocked the down-regulation of EGFR mRNA expression. Compared with the control group, A549 cells in RNAi group and erlotinib group showed increased apoptosis, reduced EGFR expression, decreased Bcl-2 expression, increased Bax protein expression, and decreased Bcl-2 / Bax ratio. Conclusion Blockade of EGFR signaling pathway may promote the apoptosis of lung adenocarcinoma A549 cells. The mechanism may be related to the decrease of Bcl-2 / Bax ratio.