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用最小二乘法拟合的多项式作为描述保留值或峰宽与流动相组成间关系的数学模型,以影响水溶性维生素分离的主要因素(流动相中有机调节剂甲醇的浓度)作为主变量,分析时间最短为目标,各最难分离物质对的分离度≥1.5为约束条件,在一定变量范围内对流动相组成进行了优化,并运用此优化方法选择了维生素 C、烟酸、烟酰胺、维生素 B_1、维生素 B_2和维生素 B_6六种水溶性维生素的最佳分离条件.
The polynomial fitted by the least square method was used as the mathematical model to describe the relationship between the retention value or the peak width and the mobile phase composition. The main factor influencing the separation of water-soluble vitamins (the concentration of organic regulator methanol in the mobile phase) was analyzed as the main variable The shortest time as the goal, the separation of the most difficult to separate material pairs ≥ 1.5 for the constraints, the mobile phase composition is optimized within a certain variable range, and use this optimization method to select the vitamin C, niacin, niacinamide, vitamins B_1, vitamin B_2 and vitamin B_6 six kinds of water-soluble vitamins the best separation conditions.