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目的:本研究旨在探讨早期干预及康复训练对宫内感染致脑损伤仔鼠脑组织神经突起生长因子neuritin表达的影响。方法:30只Wistar大鼠在受孕后第17天和18天连续2天腹腔注射脂多糖(LPS)420ug/kg.d作为模型组,10只受孕大鼠在相同日龄注射等量无菌生理盐水作为对照组,随机选取模型组仔鼠100只,分为干预组(T)50只,非干预组(NT)50只;随机选取对照组仔鼠50只为NS组。干预组进行早期干预(触摸和丰富环境)和康复训练,非干预组和NS组不给任何干预。在仔鼠出生后24小时内随机选取模型组和对照组各5只行胎盘HE染色,观察胎盘病理变化情况,3组分别在1天、7天、14天、21天、28天随机选取10只取仔鼠脑组织行HE染色观察脑组织病理变化和免疫组化染色观察脑组织neuritin的表达情况。分别于14天、21天、28天行改良BBB评定和悬吊实验测定评分。结果:LPS组较NS组悬吊实验和改良BBB得分及neuritin表达均显著降低(p<0.05),其中T组显著高于NT组(p<0.05)。结论:早期干预后可提高脑neuritin的表达并能改善其运动功能。
Objective: The purpose of this study was to investigate the effects of early intervention and rehabilitation training on the neuritin expression in brain tissue of neonatal rats with intrauterine infection. Methods: Thirty Wistar rats were intraperitoneally injected with lipopolysaccharide (LPS) at 420ug / kg.d on the 17th day and the 18th day after the conception as the model group. Ten pregnant rats were injected with the same amount of sterile physiology Saline as a control group, 100 model rats were randomly selected and divided into intervention group (T) 50, non-intervention group (NT) 50; randomly selected control group of 50 offspring NS group. The intervention group conducted early intervention (touch and rich environment) and rehabilitation training, without any intervention in non-intervention group and NS group. The placenta was randomly selected from the model group and the control group within 24 hours after birth to observe the pathological changes of the placenta. The three groups were randomly selected 10 days, 7 days, 14 days, 21 days and 28 days respectively The brain tissue of neonatal rats was examined only for pathological changes by HE staining and immunohistochemical staining for neuritin expression. Respectively in the 14th, 21st, 28th day improved BBB assessment and suspension test score. Results: Compared with NS group, the suspension test, modified BBB score and neuritin expression in LPS group were significantly decreased (p <0.05), of which the T group was significantly higher than the NT group (p <0.05). Conclusion: Early intervention can improve the expression of neuritin and improve its motor function.