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目的探讨全反式维甲酸（ＡＴＲＡ）诱导治疗急性早幼粒细胞白血病（ＡＰＬ）时，白细胞介素６（ＩＬ６）及可溶性糖蛋白１３０（ｓｇｐ１３０）、白细胞介素８（ＩＬ８）及Ａ型受体（ＩＬ８ＲＡ）变化的临床意义。方法用Ｅｌｉｓａ法动态检测血浆及骨髓单个核细胞培养上清ＩＬ６、ｓｇｐ１３０、ＩＬ８水平，用间接免疫荧光法（ＦＡＣＳ）检测体外诱导后骨髓单个核细胞（ＭＮＣ）膜ＩＬ８ＲＡ阳性率。结果初诊ＩＬ６、ｓｇｐ１３０、ＩＬ８水平明显高于正常人，ＩＬ６、ｓｇｐ１３０与白细胞（ＷＢＣ）数量呈正相关，ＩＬ８与体温呈正相关（Ｐ＜０．０５）；ＭＮＣ体外诱导７２小时，上清ＩＬ６变化不显著，ｓｇｐ１３０略下降，ＩＬ８明显下降，ＭＮＣ膜ＩＬ８ＲＡ阳性率升高；治疗中ＩＬ６与ＷＢＣ数量、早幼粒细胞绝对数相关，间歇服用ＡＴＲＡ组ＩＬ６峰值、ＷＢＣ峰值及达峰值的时间低于持续组；完全缓解者ｓｇｐ１３０明显下降，未缓解者无明显变化；ＩＬ８与中幼粒绝对数及体温相关，感染时比ＩＬ６升高更敏感。结论ＩＬ６、ｓｇｐ１３０、ＩＬ８水平可反映ＡＴＲＡ反应性，预测高白细胞血症及感染，ｇｐ１３０信号传导途径可能参与ＡＴＲＡ的诱导分化?
Objective To investigate the effects of all-trans retinoic acid (ATRA) in the treatment of acute promyelocytic leukemia (APL) with interleukin 6 (IL-6) and soluble glycoprotein 130 (sgp130) and interleukin-8 (IL-8) The clinical significance of the change of type A receptor (IL-8RA). Methods The levels of IL6, sgp130, and IL8 in culture supernatants of plasma and bone marrow mononuclear cells were detected dynamically by Elisa method. IL8RA positive in bone marrow mononuclear cells (MNC) membranes was detected by indirect immunofluorescence assay (FACS). rate. Results The levels of IL6, sgp130 and IL8 were significantly higher than those of normal subjects. IL6 and sgp130 were positively correlated with the number of white blood cells (WBCs). IL8 was positively correlated with body temperature (P<0.05); MNCs were induced in vitro. At 72 hours, the change of IL-6 in the supernatant was not significant, sgp130 was slightly decreased, IL-8 was significantly decreased, and the positive rate of IL-8RA in MNC membrane was increased; IL-6 was associated with the number of WBC and the absolute number of promyelocytic cells during treatment. The time of IL-6 peak, WBC peak and peak value in taking ATRA group was lower than that in continuous group; sgp130 in complete remission group was significantly decreased, and no significant change was observed in patients without remission; IL-8 was related to the absolute number of middle and young grains and body temperature, and it was lower than that in infection. IL-6 increased more sensitive. Conclusion The levels of IL-6, sgp130 and IL-8 can reflect the reactivity of ATRA, predicting hyper-leukemia and infection, and gp130 signaling pathway may be involved in the differentiation of ATRA.