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目的 :观察不同培养条件下变形链球菌代谢产生活性氧的情况 ,研究变形链球菌活性氧代谢的酶学基础 ,探讨变形链球菌活性氧代谢产物对菌斑微生态的作用。方法 :采用化学发光法检测变形链球菌培养液中超氧化物歧化酶 (superoxidedismutase ,SOD)含量 ;采用电子自旋捕捉法检测不同培养条件下变形链球菌产生活性氧的情况及还原型尼克酰胺腺嘌呤二核苷酸 (nicotinamideadeninedinucleotidereduced ,NADH)对次黄嘌呤—黄嘌呤氧化酶体系和Fe+ + —H2 O2 体系产生活性氧的影响。结果 :在生理盐水培养条件下 ,变形链球菌培养液中能检测到特征性的二甲基吡咯氮氧化物 (dimethylpyridineN -oxide ,DMPO) -O2 ·和DMPO -OH·的电子自旋共振 (elec tronspinresonance ,ESR)信号 ;外源性糖使变形链球菌培养过程中产生的特征性ESR信号增强 ;美兰则使ESR信号消失 ;NADH使得次黄嘌呤—黄嘌呤氧化酶体系和Fe+ + —H2 O2 体系的ESR信号明显减弱甚至消失 ;在变形链球菌培养液中检测到SOD。结论 :不同培养条件下变形链球菌代谢产生O2 ·和OH·的量不同 ,NADH氧化酶催化的反应可能是变形链球菌产生活性氧的主要来源。
OBJECTIVE: To observe the production of reactive oxygen species (ROS) in Streptococcus mutans under different culture conditions, and to study the enzymology basis of active oxygen metabolism of Streptococcus mutans and to explore the effect of metabolites of active oxygen metabolites of Streptococcus mutans on plaque microecology. Methods: The content of superoxide dismutase (SOD) in Streptococcus mutans culture medium was detected by chemiluminescence method. The production of reactive oxygen species (ROS) in Streptococcus mutans was detected by electron spin-trapping method and adenine Effect of nicotinamide-substitutedinucleotidereduced (NADH) on the production of reactive oxygen species from hypoxanthine-xanthine oxidase system and Fe + + -H2O2 system. RESULTS: The characteristic spinodal resonances of dimethylpyridine-N-oxide (DMPO) -O2 · and DMPO-OH · were detected in the culture medium of S. mutans under physiological saline conditions (elec tricalpinresonance (ESR) signal. Exogenous sugars enhanced the characteristic ESR signal produced by Streptococcus mutans culture, while the disappearance of ESR signal was induced by Meilan. NADH induced hypoxanthine-xanthine oxidase system and Fe + + -H 2 O 2 The system of ESR signal significantly weakened or even disappeared; detected in the S. mutans culture medium SOD. CONCLUSION: The amount of O2 · and OH · produced by S. mutans is different under different culture conditions. The reaction catalyzed by NADH oxidase may be the main source of active oxygen produced by Streptococcus mutans.