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已描述可靠的小肠结肠炎耶尔森氏菌(简称耶氏菌)的毒力物质是40～50巨道尔赖的质粒,这种菌具有自家凝集、钙依赖和刚果红吸收的性质。著者报告修改的草酸盐琼脂的刚果红-草酸镁琼脂(CRMOX)更易检测小肠结肠炎耶氏菌致病性,其方法如下:CRMOX 培养基:肤蛋白酶大豆琼脂40g,加825ml 蒸馏水,混合,高压121℃15分钟。溶化后,冷却至55℃,加入以下溶液:0.25M草酸钠80ml,0.25M 氧化镁80ml,20%D-半乳糖10ml 和1%刚果红5ml。半乳糖溶液高压115℃10分钟灭菌,其
The virulence substance of Yersinia enterocolitica, which has been described as reliable, is a plasmid of 40 to 50 gaultrel having properties of self-agglutination, calcium dependence and congo red absorption. The authors report that the modified oxalate agar Congo red-magnesium oxalate agar (CRMOX) is more susceptible to the pathogenicity of Y. enterocolitica as follows: CRMOX medium: Protease Soy agar 40 g, plus 825 ml distilled water, mixed, High pressure 121 ° C for 15 minutes. After dissolution, the mixture was cooled to 55 ° C and the following solutions were added: 0.25 M sodium oxalate 80 mL, 0.25 M magnesium oxide 80 mL, 20% D-galactose 10 mL, and 1% Congo red 5 mL. The galactose solution was autoclaved at 115 ° C for 10 minutes and sterilized