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利用多重PCR结合DHPLC技术,建立了高通量快速筛选检测转基因大豆及其食品的方法,确立了转基因大豆品系鉴定方法。该筛选方法的检测灵敏度为0.078 ng.μL-1,质粒检测灵敏度为1×103拷贝.μL-1。利用该方法对转基因大豆GT-40-3-2、Mon89788、A5704-12和大豆食品曲奇饼干、含大豆的调味粉、干豆腐及非转基因黑大豆进行验证,效果良好。所建立的PCR-DHPLC检测方法能同时快速准确的检测大豆及加工食品中转基因成分。
Using multiplex PCR combined with DHPLC technology, a method of high-throughput screening of transgenic soybean and its food was established, and the identification method of transgenic soybean lines was established. The detection sensitivity of this screening method was 0.078 ng.μL-1, and the sensitivity of plasmid detection was 1 × 103 copies.μL-1. The method was applied to validate the transgenic soybeans GT-40-3-2, Mon89788, A5704-12 and soybean food cookie, soybean-containing seasoning powder, dried tofu and non-genetically modified black soybeans. The established PCR-DHPLC detection method can simultaneously and rapidly and accurately detect the genetically modified components in soybean and processed foods.