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目的探讨加参方对心肌梗死大鼠心功能的影响及可能作用机制。方法采用结扎大鼠左冠状动脉前降支的方法建立大鼠急性心肌梗死模型,将术后24 h成活大鼠随机分为假手术组(19只)、模型组(30只)、加参方低剂量组(31只)、加参方高剂量组(30只)和氯沙坦组(30只)。加参方低、高剂量组分别给予加参方浸膏含生药量3、6 g/(kg·d),氯沙坦组给予氯沙坦片10 mg/(kg·d),假手术组和模型组给予相同体积蒸馏水,各组连续灌胃4周。分别于给药后1、4周末超声检测心功能,测定心肌组织缺血危险区单核-巨噬细胞计数及血管细胞黏附分子-1(VCAM-1)蛋白表达量。结果与假手术组同时间点比较,模型组大鼠心功能降低,心肌组织中单核-巨噬细胞计数及VCAM-1蛋白表达显著升高(P<0.05);与模型组同时间点比较,氯沙坦组和加参方高剂量组大鼠心功能改善明显,心肌组织单核-巨噬细胞计数和VCAM-1表达降低(P<0.05)。结论加参方可能通过抑制大鼠心肌组织单核-巨噬细胞的浸润及VCAM-1蛋白表达,从而改善心室重构,提高心功能。
Objective To investigate the effect of Jia Shen Fang on cardiac function in myocardial infarction rats and its possible mechanism. Methods The model of acute myocardial infarction was established by ligating left anterior descending coronary artery in rats. The rats were randomly divided into sham-operation group (n = 19), model group (n = 30) Low-dose group (31), plus high-dose group (30) and losartan group (30). Plus low, high dose group were given ginseng extract containing crude drug dose 3,6 g / (kg · d), losartan group losartan 10 mg / (kg · d), sham operation group And the model group were given the same volume of distilled water, each group continuous gavage for 4 weeks. The cardiac function was measured by ultrasonography at 1, 4 weeks after the administration, and the monocyte-macrophage counts and the expression of VCAM-1 protein in myocardial ischemic risk area were determined. Results Compared with the sham-operation group, the cardiac function of the model group was decreased, the number of monocyte-macrophage and the expression of VCAM-1 protein in myocardial tissue were significantly increased (P <0.05), compared with the model group , Heart function of rats in losartan group and Jia Shen Fang high dose group were significantly improved, monocyte-macrophage count and VCAM-1 expression in myocardium were decreased (P <0.05). Conclusion Ginseng prescription may improve ventricular remodeling and cardiac function by inhibiting infiltration of monocyte-macrophage and expression of VCAM-1 in rat myocardium.