论文部分内容阅读
利用栀子脂溶性蛋白作为免疫原免疫BALB/c小鼠,取其脾细胞与SP2/0细胞进行融合,通过间接ELISA方法筛选获得1株抗栀子蛋白的单克隆抗体2H8。该单克隆抗体亚类鉴定为IgG2b,轻链为Kappa链。2H8腹水效价为1∶204 800。Western blot试验证实,2H8能与栀子蛋白反应,识别相对分子质量为58.5 kDa左右的特异性蛋白质条带。以2H8腹水作为一抗建立了斑点酶联免疫吸附试验方法(Dot-ELISA),最小检出量为19.5 ng。本研究为快速检测热毒宁注射液蛋白的试剂盒的研制奠定了基础。
BALB / c mice were immunized with Gardenia lipophilus protein as immunogen and their spleen cells were fused with SP2 / 0 cells. A monoclonal antibody 2H12 against gardenin was screened by indirect ELISA. The monoclonal antibody subclass was identified as IgG2b and the light chain was Kappa chain. 2H8 ascites titer of 1: 204 800. Western blot confirmed that 2H8 reacted with Gardenia protein and identified a specific protein band with a relative molecular mass of 58.5 kDa. Dot blot was developed using 2H8 ascites as the primary antibody with a minimum detection of 19.5 ng. This study lays a foundation for the rapid development of the kit for the determination of Rendynin injection protein.