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本文报道呋喃氟脲嘧啶(FT-207)血清浓度的反相高效液相色谱测定法。固定相为Shim-packCLC-ODS柱(0.15m×6.0φ),流动相为定时程序控制的甲醇溶液,检测波长254nm。样品用甲醇沉淀蛋白,取上清液20ul直接进样。最低检测浓度为0.03μg/ml,标准曲线的线性范围为0.03～132.0μg/ml(r=0.99997)。用本法测定了9只大鼠口服FT-207后的血清浓度,其药时曲线符合一室开放模型。用残数法算得主要药代学参数为k(?)=1.2883±1.0972h~(-1),k_e=0.0767±0.0179h~(-1),tal/2=0.77±0.37h,tel/2=9.42±1.86h,t_p=2.88±1.02h,C_(max)=83.56±11.87μg/ml,AUC=1659.43±258.48μg.h/ml。
In this paper, a reverse-phase high performance liquid chromatography (HPLC) method was developed for the determination of the serum concentration of furanfluracil (FT-207). The stationary phase was a Shim-pack CLC-ODS column (0.15m × 6.0φ). The mobile phase consisted of a time-programmed methanolic solution with a detection wavelength of 254nm. Sample precipitation of protein with methanol, the supernatant 20ul direct injection. The lowest detection concentration was 0.03 μg / ml. The calibration curve showed a linear range of 0.03 ~ 132.0 μg / ml (r = 0.99997). The serum concentrations of FT-207 after oral administration of 9 rats were determined by this method. The drug-drug curve accords with the open-chamber model. The main pharmacokinetic parameters calculated by the residual number method were k (?) = 1.2883 ± 1.0972 h -1, k_e = 0.0767 ± 0.0179 h -1, tal / 2 = 0.77 ± 0.37 h, tel / 2 = 9.42 ± 1.86 h, t_p = 2.88 ± 1.02 h, C max = 83.56 ± 11.87 μg / ml, AUC = 1659.43 ± 258.48 μg.h / ml.