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选取淋巴细胞分类>50％的渗出性胸水患者,年龄≥15岁,剔除已经接受抗结核治疗或伴有其它疾病胸膜炎病史的患者。用肝素化注射器采取胸水标本40ml,开始20ml进行细胞计数与分类、抗酸染色、细胞学检查、蛋白和乳酸脱氢酶测定。后20ml经3000rpm离心15min。取上清液置-70℃条件下冷藏。胸水和胸膜活检标本采用双份Ogawa培养基进行抗酸杆菌培养共8周。IFN-γ测定采用ELISA药盒(其敏感度可检出低至3pg/ml的水平)。 结核性胸水的诊断要求符合以下条件之一:①自胸水或
Patients with> 50% exudative pleural effusion with a lymphocyte classification of> 15 years were excluded from the group who had already received anti-TB therapy or a history of other diseases with pleuritis. Using a heparinized syringe, 40 ml of pleural effusion samples were taken and 20 ml of cells were counted and classified for cell counting, acid-fast staining, cytology, and protein and lactate dehydrogenase assays. After 20ml centrifugation at 3000rpm 15min. The supernatant was stored at -70 ℃ for storage. Pleural effusion and pleural biopsy specimens were treated with acid-fast bacilli in duplicate Ogawa media for 8 weeks. IFN-γ assay using ELISA kit (the sensitivity can be detected as low as 3pg / ml level). Tuberculosis pleural effusion diagnosis requirements meet one of the following conditions: ① since the pleural effusion or