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目的 :探讨乙肝患者治疗过程中e抗原 -抗体系统的变化与HBV复制的关系。方法 :运用荧光定量PCR技术对 164例血清HBeAg( +)的乙肝患者治疗过程中HBV -DNA血清水平进行监测。结果 :治疗前HBeAg( +)患者HBV DNA阳性率为98 2 %。治疗过程中 ,97例出现HBeAg( -) /抗 -HBc( +) ,此时HBV -DNA阳性率为 48 5 %;75例出现HBeAg( -) /抗 -HBe( +) ,其HBV DNA检出率为 3 2 0 %;42例出现抗 HBe( +) /抗 HBc( +) ,其HBV DNA检出率为 7 1%;3 0例出现抗 HBe( +) /抗 HBs( +) (包括抗 HBe( +) /抗 HBe( +) /抗 HBc( +) ) ,其HBV DNA检出率为 6 7%;在 7例抗 HBc( +)及 5例全阴的标本中 ,也有HBV DNA检出。结论 :在乙肝的治疗过程中血清HBeAg的消失及抗 HBe的产生 ,标志着HBV复制水平的下降 ,了解各阶段病毒复制程度对乙肝治疗及疗效评价有重要意义
Objective: To investigate the relationship between e antigen-antibody system changes and HBV replication in the treatment of hepatitis B patients. Methods: HBV-DNA serum levels in 164 patients with HBeAg (+) hepatitis B during treatment were monitored by real-time fluorescence quantitative PCR. Results: The positive rate of HBV DNA in HBeAg (+) patients before treatment was 98 2%. In the course of treatment, HBeAg (-) / anti-HBc (+) was detected in 97 cases, and the positive rate of HBV DNA was 48.5% at the same time. HBeAg (-) / anti-HBe The rate of HBV DNA was 71.0% in 42 patients with anti-HBe (+) / anti-HBc (+), and the anti-HBe Including anti-HBe (+) / anti-HBe (+) / anti-HBc (+)). The detection rate of HBV DNA was 67%. Among the 7 anti-HBc DNA detection. Conclusions: The disappearance of serum HBeAg and the anti-HBe production in the course of hepatitis B treatment indicate the decrease of HBV replication level. It is of great significance to understand the extent of virus replication in each stage of hepatitis B treatment and efficacy evaluation