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目的:建立HPLC法同时测定红大戟药材中芦西定、5-羟基巴戟醌与红大戟素的含量。方法:采用Waters Xbridge C18色谱柱(250 mm×4.6 mm,5μm),以0.05%磷酸为流动相A,以乙腈为流动相B,梯度洗脱;流速为1.0 ml·min~(-1),检测波长为280 nm,柱温为30℃。结果:芦西定、5-羟基巴戟醌、红大戟素的线性范围分别为0.147~29.400μg·ml~(-1)(r=0.999 6)、0.126~25.200μg·ml~(-1)(r=0.999 9)、0.135~27.000μg·ml~(-1)(r=0.999 5),平均加样回收率分别为98.50%(RSD=1.20%)、98.72%(RSD=0.73%)、101.10%(RSD=1.12%)(n=6)。结论:本文建立的方法经方法学验证可用于评价红大戟药材质量。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of LuXidine, 5-Hydroxy-Benzoquinone and Eupestanin in Radix Euphorbia. METHODS: Waters Xbridge C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of 0.05% phosphoric acid as mobile phase A and acetonitrile as mobile phase B at a flow rate of 1.0 ml · min -1. The detection wavelength was 280 nm and the column temperature was 30 ℃. Results: The linear ranges of the two compounds were 0.147-29.400μg · ml -1 (r = 0.999 6), 0.126-25.25μg · ml -1 (r = 0.999 9) and 0.135 ~ 27.000 μg · ml -1 (r = 0.999 5) respectively. The average recoveries were 98.50% (RSD = 1.20%) and 98.72% (RSD = 0.73% , 101.10% (RSD = 1.12%) (n = 6). Conclusion: The method established in this paper can be used to evaluate the quality of Radix Euphorbiae by methodological verification.