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本研究对新疆乌鲁木齐、石河子、奎屯农贸市场以及超市的肉品、蔬菜、乳制品和即食食品中的大肠杆菌进行检测,从198份样品中分离出63株大肠杆菌。采用K-B法,对63株大肠杆菌进行17种抗生素敏感试验;采用PCR技术检测9种耐药基因。药敏结果表明,63株大肠杆菌对四环素(44.44%)、氨苄西林(39.68%)和萘啶酮酸(38.10%)耐药率较高,所有受试菌株对亚胺培南(0.00%)敏感性最强;肉品、蔬菜分离株对四环素(57.14%、52.94%)耐药性最强,乳源性分离株对氨苄西林(26.67%)耐药性最强,即食食品分离株对17种抗生素敏感;乌鲁木齐、奎屯、石河子分离株对四环素(65.00%、40.00%、32.14%)耐药率最高。PCR结果表明,耐药菌株中,sul2耐药基因检出率最高(75.00%),add B耐药基因的检出率最低(19.23%)。1重以上耐药菌株占总菌数的63.49%,3重以上耐药菌株占总菌数的39.68%。新疆地区食源性大肠杆菌多重耐药性比较严重。
In this study, E. coli in meat products, vegetables, dairy products and ready-to-eat foods from Urumqi, Shihezi, Kuitun farmers markets and supermarkets in Xinjiang were detected. 63 strains of E.coli were isolated from 198 samples. K-B method was used to test 17 kinds of antibiotics in 63 Escherichia coli strains. Nine kinds of resistance genes were detected by PCR. The results of drug susceptibility showed that 63 strains of Escherichia coli had a high resistance rate to tetracycline (44.44%), ampicillin (39.68%) and nalidixic acid (38.10%), all tested strains were resistant to imipenem (0.00% The sensitivity of tetracycline (57.14%, 52.94%) was the strongest among the meat and vegetable isolates, and the most resistant to ampicillin (26.67% Antibiotics sensitive; Urumqi, Kuitun, Shihezi tetracycline strains (65.00%, 40.00%, 32.14%) the highest rate of resistance. PCR results showed that the detection rate of sul2 resistance gene was the highest (75.00%) in drug-resistant strains and the lowest was add B resistance gene (19.23%). More than 1 drug-resistant strains accounted for 63.49% of the total number of bacteria, 3 or more resistant strains accounted for 39.68% of the total number of bacteria. Multi-drug-resistant Escherichia coli in Xinjiang is more serious.