论文部分内容阅读
目的分析习惯性自发性流产女性和正常女性的染色体结构稳定性的不同,为提高生育质量提供理论依据。方法随机选择习惯性自发性流产的女性20人为观察组及正常的育龄女性20人为对照组,对其外周血淋巴细胞按姐妹染色单体互换(SCE)检测方法、微核检测方法及染色体断裂检测方法进行培养检测,分别记数SCE发生率、微核出现率及染色体断裂发生率。结果观察组的外周血淋巴细胞SCE发生率和微核出现率明显高于对照组(P<0.05);观察组的外周血淋巴细胞的染色体断裂发生率与对照组比较差异不明显(P>0.05)。结论SCE发生率和微核出现率可以作为经常暴露在易致DNA损伤的环境下发生习惯性自发性流产女性染色体结构稳定性的检测指标。
Objective To analyze the differences in the structural stability of chromosome between habitual spontaneous abortion women and normal females, and to provide a theoretical basis for improving reproductive quality. Methods 20 randomly selected women with habitual spontaneous abortion and 20 normal women of childbearing age were selected as the control group. Their peripheral blood lymphocytes were analyzed by the methods of sister chromatid exchange (SCE), micronucleus test and chromosome breakage Detection methods for culture detection, respectively, count the incidence of SCE, micronuclei and chromosomal breakup incidence. Results The incidences of SCE and micronuclei in peripheral blood lymphocytes in the observation group were significantly higher than those in the control group (P <0.05). The incidence of chromosomal rupture in peripheral blood lymphocytes in the observation group was not significantly different from that in the control group (P> 0.05 ). Conclusion The incidence of SCE and the incidence of micronuclei can be used as indicators of the structural stability of female spontaneous spontaneous abortion under the conditions of frequent exposure to DNA damage.