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目的 探讨γ 谷氨酰转移酶 (GGT)mRNA亚型的转化与原发性肝癌 (HCC)发生的关系 ,寻找肝癌早期诊断的新方法。方法 以逆转录聚合酶链反应方法检测正常对照组、非癌肝病组、肝癌组及肝转移癌肝组织及外周血的 3种GGTmRNA亚型 (A、B、C亚型 )。结果 正常肝组织、非癌肝病的肝组织及肝转移癌癌周组织主要的GGTmRNA类型为A亚型 ,肝癌组织、癌旁组织及远癌组织GGTmRNA B亚型的阳性率显著高于正常肝脏及非癌肝病的肝组织 (P <0 .0 5 ) ,肝癌组织GGTmRNA A亚型阳性率明显低于正常对照及非癌肝病组 (P <0 .0 5 )。在 2 6例HCC中有 12例外周血中检出GGTmRNA B亚型 ,甲胎蛋白阴性的 10例HCC中有 5例检出GGTmRNA B亚型。结论 GGTmRNA亚型转化与肝癌发生有密切关系 ,分析GGT基因可望成为监测肝细胞癌变的灵敏方法。
Objective To investigate the relationship between the transformation of γ-glutamyl transferase (GGT) mRNA subtypes and the occurrence of primary hepatocellular carcinoma (HCC) in order to find out a new method for the early diagnosis of liver cancer. Methods Three GGT mRNA subtypes (A, B, C subtypes) of normal control group, non-cancerous liver disease group, liver cancer group, liver metastasis liver cancer group and peripheral blood were detected by reverse transcription-polymerase chain reaction. Results The positive rates of GGT mRNA in the normal liver tissues, non-cancerous liver tissues and metastatic liver cancer tissues were A subtypes. The positive rates of GGT mRNA subtype B in HCC tissues, paracancerous tissues and distant cancer tissues were significantly higher than those in normal liver and The positive rates of GGT mRNA A subtypes in non-cancerous liver tissues were significantly lower than those in normal controls and non-cancerous liver tissues (P <0.05). GGT mRNA subtype B was detected in 12 peripheral blood samples of 26 HCC cases and 5 of 10 alpha-fetoprotein negative HCC samples. Conclusion The transformation of GGT mRNA subtype is closely related to the occurrence of hepatocellular carcinoma. Analyzing the GGT gene is expected to become a sensitive method to monitor the carcinogenesis of hepatocellular carcinoma.