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黑果枸杞和宁夏枸杞为枸杞属中两种重要的药用植物,具有极大的药用价值。本研究利用转录组数据克隆了黑果枸杞和宁夏枸杞黄酮醇合酶基因(Flavonol synthase,FLS),分别命名为LrFLS及LbFLS,并通过实时定量PCR(real-time quantitative PCR)分析其在两种枸杞果实四个发育时期中的表达模式。研究结果表明:LrFLS与LbFLS各编码一条由350个氨基酸残基组成的蛋白序列。蛋白序列分析表明:LrFLS、LbFLS相似度高达99%,各含有两个保守的功能域。进化分析表明,LrFLS和LbFLS同属茄科的烟草、马铃薯的FLS蛋白序列相似度最高。实时定量PCR结果表明,LrFLS在S2时期的表达量最高,在果实发育过程中呈现先升高后降低的趋势;而LbFLS在S2时期表达量最低,呈现先降低后升高的趋势。本研究为揭示黄酮醇在黑果枸杞和宁夏枸杞果实中的累积具有一定的启示作用。
Black fruit Chinese wolfberry and Chinese wolfberry medlar are two important medicinal plants, with great medicinal value. In this study, Flavonol synthase (FLS) of Lycium barbarum and Lycium barbarum were cloned by using transcriptome data and named as LrFLS and LbFLS respectively. The results of real-time quantitative PCR Expression Patterns of Lycium Fruit in Four Developmental Stages. The results showed that: LrFLS and LbFLS each encode a protein sequence consisting of 350 amino acid residues. Protein sequence analysis showed that the similarities of LrFLS and LbFLS were as high as 99%, each containing two conserved domains. Phylogenetic analysis showed that both LrFLS and LbFLS shared the same FLS protein sequence identity in potato and potato. The results of real-time PCR showed that LrFLS had the highest expression in S2 and increased first and then decreased in fruit development, while LbFLS had the lowest expression and then increased in S2. This study is to reveal the accumulation of flavonols in black fruit of Lycium barbarum and Lycium barbarum fruit has some enlightenment.