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目的 研究雌激素在诱发实验性自身免疫性甲状腺炎 (EAT)形成中的影响。方法 10周龄Wistar大鼠进行双侧卵巢切除术后采用同种属SD大鼠甲状腺球蛋白 (Tg)免疫诱发EAT。病理切片HE染色观察甲状腺炎症反应。采用放免方法测定血清中E2 、PRL水平。间接酶联免疫吸附实验 (ELISA )测定血清Tg抗体 (TgAb)水平。逆转录 聚合酶链反应 (RT PCR)检测甲状腺组织中γ 干扰素 (IFN γ)、白细胞介素 10 (IL 10 )mRNA的表达水平。结果 大鼠切除双侧卵巢后 ,其血清中雌二醇 ( 17.60± 1.0 2 )pmol/L和PRL( 2 .45± 0 .15 ) μg/L水平明显低于未切除卵巢组〔分别为 ( 5 8.5 6± 6.99) pmol/L和 ( 3 .2 2± 0 .17) μg/L〕(均P <0 .0 1) ,实验性自身免疫性甲状腺炎的发病率和甲状腺组织中炎细胞浸润程度也均较未切除卵巢直接诱发EAT组明显减轻 ,甲状腺组织中IFN γmRNA的表达水平 ( 0 .87± 0 .0 3 )较未切除卵巢直接诱发EAT组 ( 0 .99± 0 .0 0 )明显下降 (P <0 .0 5 ) ,而IL 10mRNA的表达水平 ( 0 .98± 0 .0 1)则高于未切除卵巢直接诱发EAT组 ( 0 .83± 0 .0 5 )。诱发为EAT的两组大鼠血清TGAb平均水平 ( 1.3 9± 0 .0 3 )明显高于非诱发EAT对照组的平均水平 ( 0 .18± 0 .0 0 )(P <0 .0 1)。结论 雌激素水平低下可
Objective To study the effect of estrogen on the induction of experimental autoimmune thyroiditis (EAT). Methods Eighteen Wistar rats were immunized with Thyroglobulin (Tg) of SD rats of the same genus after bilateral ovariectomy. Pathological sections were observed by HE staining for thyroid inflammation. Serum E2 and PRL levels were determined by radioimmunoassay. Indirect enzyme-linked immunosorbent assay (ELISA) was used to determine serum Tg antibody (TgAb) levels. The levels of IFN-γ and IL-10 mRNA in thyroid tissue were detected by RT-PCR. Results The levels of estradiol (17.60 ± 1.02) pmol / L and PRL (2.45 ± 0.15) μg / L in the ovariectomized rats were significantly lower than those in the ovariectomized groups [( 5 8.5 6 ± 6.99) pmol / L and (3.22 ± 0.17) μg / L〕 (all P <0.01), the incidence of experimental autoimmune thyroiditis and inflammatory cells in thyroid tissue The infiltration degree was also significantly lower than that of the EAT group which was directly induced by the ovariectomized group. The expression level of IFN-γmRNA in the thyroid tissue (0.87 ± 0.30) was significantly higher than that in the EAT group (0.99 ± 0. 0 0 ) Was significantly decreased (P <0.05), while the expression level of IL 10 mRNA (0 .98 ± 0. 01) was higher than that of the EAT group (0.83 ± 0.05) induced by ovariectomy. The mean serum TGAb level of two groups of rats induced by EAT (1.3 9 ± 0.30) was significantly higher than that of non-induced EAT control group (0.18 ± 0. 0 0) (P <0.01) . Conclusions Estrogen levels are low