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为探讨不同施氮水平对烤烟质体色素含量和代谢途径基因的影响,通过使用电镜技术、荧光定量PCR和液相色谱法,研究了在不同施氮水平处理条件下,中部叶成熟过程中的叶绿体超微结构,质体色素含量和质体色素代谢关键基因的表达模式。结果表明:随着施氮量的增加,叶绿体被膜上的嗜锇颗粒增多增大,类囊体片层的数量增加,淀粉颗粒数量增加,体积增大;施氮量的增加处理可促进叶绿素和类胡萝卜素的合成,进而促进质体色素的合成;质体色素代谢相关基因的表达模式分析显示,PSY和CHL基因的转录水平受氮素调控,且在高氮水平下抑制表达,是质体色素代谢途径中的关键基因。
In order to investigate the effects of different nitrogen levels on the plastid pigment content and metabolic pathway genes in flue-cured tobacco, the effects of nitrogen fertilization on the plastid pigment content and metabolic pathway genes were studied by using electron microscopy, fluorescence quantitative PCR and liquid chromatography. Chloroplast ultrastructure, plastid pigment content and plastid pigment metabolism key gene expression patterns. The results showed that with the increase of nitrogen application, the osmiophilic particles on the chloroplast membrane increased, the number of thylakoid membrane increased, the number of starch granules increased and the volume increased. The nitrogen application increased the chlorophyll and Carotenoid synthesis, and then promote the synthesis of plastid pigment; plastid pigment metabolism-related gene expression pattern analysis showed that PSY and CHL gene transcription level is regulated by nitrogen and inhibited expression at high nitrogen levels, is the plastid Key genes in the pigment metabolism pathways.