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探讨了蜂胶对人急性髓细胞性白血病细胞株HL-60细胞增殖的抑制作用及诱导分化活性。方法:①巴西产蜂胶块用水或95%乙醇提取,分别制备水提取蜂胶(WEP)和乙醇提取蜂胶(EEP)。②以四唑氮蓝(NBT)还原试验以及流式细胞仪检测表面抗原CD11b表达为指标观察诱导分化活性。③通过Hoechst
The inhibitory effect of propolis on the proliferation of human acute myeloid leukemia cell line HL-60 cells and its differentiation activity were investigated. Methods: 1 Brazilian propolis blocks were extracted with water or 95% ethanol. Water extraction propolis (WEP) and ethanol extraction propolis (EEP) were prepared. 2 The tetrazolium blue (NBT) reduction test and flow cytometry were used to detect the expression of surface antigen CD11b as an index to observe the induction of differentiation activity. 3 through Hoechst