论文部分内容阅读
叶绿素是调节光合作用的关键色素,对籽粒形成有着重要作用。本研究以美国半矮秆大豆Charleston为母本,东北地区主栽品种东农594为父本杂交衍生的147个重组自交系群体为材料,基于经SLAF测序获得的大豆高密度遗传图谱,利用复合区间作图法(CIM)、多重区间作图法(MIM)和完备区间作图法(ICIM)对大豆叶绿素含量进行QTL联合定位分析,并结合大豆基因组基因注释信息对QTL区段内的候选基因进行预测。利用CIM算法定位出2个QTL,表型遗传贡献率分别为6%和9.3%。利用MIM算法定位到了1个QTL,表型遗传贡献率为8.1%。利用ICIM算法定位到了1个QTL,表型遗传贡献率为7.76%。其中qchl-G-1被CIM和MIM两种算法同时检测到。在上述3个QTL区段内共含有151个基因,根据大豆基因组基因注释信息,筛选到了3个与叶绿素相关的候选基因,这些结果为叶绿素含量的遗传剖析和标记辅助育种提供理论基础,有利于分子辅助育种的发展。
Chlorophyll is a key pigment regulating photosynthesis and plays an important role in grain formation. In this study, 147 recombinant inbred lines derived from the Charleston semi-dwarf soybean as the female parent and Dongnong 594 as the male parent of the northeast China were used as materials. Based on the high-density soybean genetic map obtained by SLAF sequencing, QTL combined mapping of soybean chlorophyll content with composite interval mapping (CIM), multiple interval mapping (MIM) and complete interval mapping (ICIM), combined with soybean genome annotation information, Gene prediction. Two QTLs were mapped using CIM algorithm, and the phenotypic contribution rate was 6% and 9.3% respectively. One QTL was mapped using MIM and the phenotypic contribution rate was 8.1%. One QTL was mapped using ICIM algorithm and the phenotypic contribution rate was 7.76%. Among them, qchl-G-1 is detected by both CIM and MIM simultaneously. A total of 151 genes were contained in the above three QTL segments. Three candidate genes related to chlorophyll were screened based on the gene annotation information of soybean genome. These results provided the theoretical basis for genetic analysis and marker-assisted breeding of chlorophyll content, Development of Molecular Assisted Breeding.