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目的通过回顾进行的PAS染色实验,探讨经验与体会。方法利用PAS染色试剂盒和自行配制的Schiff试剂等染色,光镜下观察并且进行分析。结果糖原颗粒呈紫红色,细胞核呈蓝色。结论影响PAS染色的原因主要在于高碘酸氧化液的浓度;Schiff试剂配置的成功与否和染色过程及孵育过程直接相关;苏木素复染细胞核。
Objective By reviewing the PAS staining experiments to explore experience and experience. Methods PAS staining kit and self-prepared Schiff reagent and other staining, light microscopy and analysis. Results glycogen particles were purple, the nucleus was blue. Conclusion The main cause of PAS staining is the concentration of periodate oxidation solution. The success or failure of Schiff’s reagent configuration is directly related to the dyeing process and incubation process. The hematoxylin counterstain the nucleus.