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目的:比较iRoot FM与传统根管消毒材料氢氧化钙[Ca(OH)_2]及三联抗生素糊剂(triple antibiotic paste,TAP)对牙髓卟啉单胞菌(Porphyromonas endodontalis,P.endodontalis)的抗菌效果,评价iRoot FM的抗菌性能,为临床上根管消毒材料的选择提供参考。方法:采用国际标准菌株P.endodontalis ATCC35406进行琼脂平板扩散实验。实验组为iRoot FM组、Ca(OH_)2组及TAP组,以蒸馏水作为空白对照组。将冻干的P.endodontalis复苏,増菌,接种于培养皿中,干燥后打孔,将材料分别加入小孔中,37℃厌氧箱培养72 h,测定抑菌环直径(mm)。实验重复6次(n=6),采用SPSS17.0软件包对数据进行统计学分析。结果:iRoot FM、Ca(OH)_2及TAP 3组抑菌环直径分别为(20.74±4.35)mm、(24.89±3.84)mm和(34.51±1.20)mm。其中,iRoot FM组与Ca(OH)_2组相比,无显著差异(P>0.05);而iRoot FM组、Ca(OH)_2组与TAP组相比,差异具有显著性(P<0.05)。对照组无抑菌环出现。结论:新型生物陶瓷材料iRoot FM在体外实验中对牙髓卟啉单胞菌具有较强的抗菌作用,提示其在根管消毒中具有良好的应用前景。
OBJECTIVE: To compare the inhibitory effect of iRoot FM and traditional root canal disinfection materials Ca (OH) 2 and triple antibiotic paste (TAP) on Porphyromonas endodontalis (P.endodontalis) Antibacterial effect, evaluate the antibacterial properties of iRoot FM, provide a reference for the choice of clinical root canal disinfection materials. Methods: The international standard strain P.endodontalis ATCC35406 agar plate diffusion test. The experimental group was iRoot FM group, Ca (OH) 2 group and TAP group, with distilled water as blank control group. The freeze-dried P.endodontalis was resuscitated, and the bacteria were inoculated into a petri dish, dried, and then punched. The materials were respectively added into the small holes and incubated in an anaerobic chamber at 37 ° C for 72 h. The diameter of the antibacterial ring was measured. The experiment was repeated 6 times (n = 6), the data were analyzed by SPSS17.0 software package. Results: The diameters of antibacterial ring in iRoot FM, Ca (OH) _2 and TAP 3 groups were (20.74 ± 4.35) mm, (24.89 ± 3.84) mm and (34.51 ± 1.20) mm, respectively. There was no significant difference between iRoot FM group and Ca (OH) 2 group (P> 0.05), while there was significant difference between iRoot FM group and Ca (OH) 2 group and TAP group (P <0.05) . No inhibition zone appeared in the control group. CONCLUSION: iRoot FM, a new bioceramic material, has strong antibacterial activity against Porphyromonas Pseudomonas in vitro, suggesting that it has good application prospect in root canal disinfection.