论文部分内容阅读
目的:了解神经酰胺信号转导在肝肿瘤细胞中的生物学意义。方法:MTT法、荧光染色、流式细胞术、琼脂糖凝胶电泳、Western blot。结果:神经酰胺对Bel7402细胞生长抑制的IC_(50)值为14.28μmol·L~(-1)。细胞核浓缩,荧光明显增强;DNA呈现典型“梯型”变化;p53蛋白表达呈下降趋势;Bcl-2蛋白表达明显降低;Bax蛋白表达无明显改变。结论:神经酰胺诱导肝肿瘤细胞凋亡,与Bcl-2表达下调有关。
Objective: To understand the biological significance of ceramide signal transduction in liver tumor cells. Methods: MTT assay, fluorescence staining, flow cytometry, agarose gel electrophoresis, Western blot. RESULTS: The IC 50 value of ceramide inhibited the growth of Bel7402 cells was 14.28 μmol·L -1 . Concentration of nuclei, fluorescence was significantly enhanced; DNA showed a typical “ladder” changes; p53 protein expression showed a downward trend; Bcl-2 protein expression was significantly reduced; Bax protein expression did not change significantly. CONCLUSION: Ceramide induces apoptosis of liver tumor cells and is related to the down-regulation of Bcl-2 expression.