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用ｃＤＭＤｓ探针，从人Ｘ染色体噬菌体文库中筛选并作ＤＮＡ印迹杂交鉴定出含有抗肌萎缩蛋白基因５１号外显子的克隆。经限制酶切图谱分析，确定ＫｐｎⅠ和ＨｉｎｄⅡ为亚克隆位点，从而获得了与抗肌萎缩蛋白基因５１号外显子相连的５０和５１号内含子亚克隆，为该区段的核苷酸顺序分析打下了基础。
Clones containing the exon 51 of the dystrophin gene were identified by screening with a cDMDs probe from a human X chromosome phage library and Southern blot hybridization. Restriction enzyme digestion analysis confirmed that Kpn I and Hind II were subcloning sites, resulting in subclones 50 and 51 linked to exon 51 of the dystrophin gene, providing the nucleotides Sequence analysis laid the foundation.