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近几年我国海水养殖鱼类发生病毒性传染病 ,病鱼鳍、鳃及体表皮肤出现乳头瘤样赘生物。将发病牙鲆(Paralichthysolivaceus) 表皮瘤组织进行超薄切片 ,电镜下在病变组织明显肥大的细胞胞浆内发现大量球状病毒 ,呈二十面体对称 ,具包膜 ,直径约 2 10nm。根据虹彩病毒主要衣壳蛋白 (majorcapsidprotein ,MCP)基因中间保守区序列设计简并引物 ,应用PCR方法从病变组织中扩增出长 6 36bp的片段。经DNA序列测定及同源性分析表明 ,该段核苷酸序列与欧洲分离的淋巴囊肿病病毒 1型 (lymphocysticdiseasevirustype 1,LCDV - 1)MCP基因相应序列有 77%的同源性 ,表明该病毒属虹彩病毒科 (Iridoviridae)淋巴囊肿病毒属 (Lymphocystivirus) ,但与欧洲分离株有一定差异。将该病毒暂命名为淋巴囊肿病病毒中国分离株 (lymphocystisdiseasevirus-Chineseisolate ,LCDV -cn)。
In recent years, China’s marine aquaculture fish epidemic of viral diseases, sick fish fin, gills and skin surface appear papillomatous neoplasm. The Paralichthys olivaceus epidermoid tissue was ultrasonically sectioned, and a large number of globular viruses were found in the cytoplasm of the paralyzed tissue under electron microscope. They were icosahedral symmetrical and enveloped with a diameter of about 210 nm. A degenerate primer was designed according to the conserved region of the major capsid protein (MCP) gene of iris virus, and a 636 bp fragment was amplified from the diseased tissue by PCR. DNA sequence analysis and homology analysis showed that the nucleotide sequence of the nucleotide sequence showed 77% homology with the corresponding sequence of MCP gene isolated from Europe, which showed that the virus Is a family of Iridoviridae Lymphocystivirus, but with the European isolates have some differences. This virus is temporarily named lymphocystisdisease virus-Chinese isolate (LCDV-cn).