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目的建立H-2半相合小鼠CD34+细胞和TK+T细胞混合移植急性GVHD模型。方法自亲代雄性代鼠骨髓分离CD34+细胞,同时取脾脏T细胞转染tk基因,两者移植雌性F1受鼠,每只输入CD34+细胞和TK+T细胞分别为1.0×105和1.0×107。单纯照射组和CD34+细胞组作对照。结果CD34++TK+T细胞组均发生急性GVHD,生存时间13.3±1.2天,受鼠死亡前外周血白细胞1.76±0.25×109/L,病理表现与单纯照射组明显不同。单纯照射组小鼠生存时间6.5±0.8天,与CD34++TK+T细胞组比较,P <0.05。CD34+细胞组和CD34++TK+T细胞组小鼠外周血检查可见Y染色体。结论H-2半相合CD34+细胞和TK+T细胞混合移植能建立急性GVHD模型。
Objective To establish an acute GVHD model of mixed H-2 haploidentical mouse CD34 + cells and TK + T cells. Methods The CD34 + cells were isolated from the bone marrow of the offspring male offspring. At the same time, the tk gene was transfected into the splenic T cells. The female F1 mice were transplanted with 1.0 × 105 and 1.0 × 107 CD34 + cells and TK + T cells respectively. Simple irradiation group and CD34 + cell group as a control. Results Acute GVHD occurred in CD34 + + TK + T cells, with a survival time of 13.3 ± 1.2 days. The number of peripheral leukocytes in preimplantation mice was 1.76 ± 0.25 × 109 / L. The pathological findings were significantly different from those in pure irradiation group. Compared with CD34 + + TK + T cells group, the survival time of mice in pure irradiation group was 6.5 ± 0.8 days, P <0.05. Y chromosome was detected in peripheral blood of CD34 + cell group and CD34 + + TK + T cell group mice. Conclusions Acute GVHD model can be established by mixed transplantation of H-2 haploidentical CD34 + cells and TK + T cells.