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目的:为了从珍贵药材野山参的少量根须提取DNA,同时尽量保持野山参形态的完整,特研究本方法。方法:我们改进了CTAB提取法,设计了一种简捷的DNA制备方案。结果:从0.001g人参根须提取DNA,得率达2250μg/g。结论:RAPD扩增效果良好,可作其他药材微量组织提取DNA的参考方法。
Objective: To study the method of extracting DNA from a small amount of roots of wild medicinal wild ginseng, while maintaining the integrity of wild ginseng morphology. Methods: We improved the CTAB extraction method and designed a simple DNA preparation protocol. RESULTS: DNA was extracted from 0.001g ginseng root and the yield was 2250μg/g. Conclusion: RAPD has good amplification effect and can be used as a reference method for extracting DNA from other herbs.